SUPELCO 59356-U Cyano Hplc Column 15X4.6MM

( Brand: Supelco ), ( Manufacturer Part Number: 59356-U ), ( Country/region Of Manufacture: Japan ), ( Bundle Listing: No )

Review SUPELCO Discovery Cyano Hplc Column 15x4.6mm

The Supelco 59356-U C18 Cyano HPLC Column is a high-performance liquid chromatography (HPLC) column specifically designed for the separation and analysis of cyanoacetic acid derivatives and other compounds containing cyano groups. This column is manufactured by Sigma-Aldrich, a leading supplier of scientific research chemicals, and is part of their extensive portfolio of HPLC columns.

The Supelco 59356-U C18 Cyano HPLC Column features a cyano, or C18-cyanopropyl, stationary phase that provides enhanced retention and selectivity for cyano-containing compounds. The 5 micron particle size offers a good balance between resolution and analysis speed, making it suitable for both routine and more complex separations.

The column measures 15 centimeters in length and 4.6 millimeters in internal diameter, providing a total packing volume of approximately 150 cubic centimeters. This size is commonly used for medium-pressure HPLC systems, which can accommodate a wide range of sample volumes and flow rates.

The stationary phase is chemically bonded to a silica gel substrate and is designed to be stable over a wide pH range, allowing for the analysis of both acidic and basic compounds. The column is also compatible with various mobile phases, including water, acetonitrile, and methanol, making it a versatile tool for a wide range of applications.

The Supelco 59356-U C18 Cyano HPLC Column is engineered for high efficiency and reproducibility, ensuring consistent and reliable results. The column comes with a guard column to protect against particle contamination and extend the life of the analytical column. Additionally, it is packed in a protective sleeve to ensure that it arrives in pristine condition.

In summary, the Supelco 59356-U C18 Cyano HPLC Column is a powerful tool for the separation and analysis of cyano-containing compounds. Its cyano stationary phase provides enhanced retention and selectivity, while its 5 micron particle size offers a good balance between resolution and analysis speed. The column is versatile, stable, and compatible with a wide range of mobile phases and pH conditions, making it a valuable addition to any HPLC laboratory.

The Supelco 59356-U C18 reversed-phasecyano HPLC column (150 mm x 4.6 mm) is a popular choice for the separation of basic and acidic compounds, particularly those containing nitro, amino, and sulfonate functional groups. Here are some pros and cons of purchasing this column:

1. High selectivity: The cyano stationary phase of this column provides excellent selectivity for separating compounds with different functional groups.

2. High efficiency: With a particle size of 5 m, this column offers high efficiency, resulting in better resolution and increased peak capacity.

3. Good reproducibility: Supelco's rigorous manufacturing process ensures consistent column performance, making it a reliable choice for routine analysis.

4. Wide pH range: The cyano column can be used over a wide pH range, making it versatile for a variety of applications.

5. Compatibility with various mobile phases: This column can be used with both aqueous and organic mobile phases, allowing for flexibility in method development.

1. Limited capacity: Due to its small diameter (4.6 mm), this column has a limited sample loading capacity, making it less suitable for high-throughput or large-scale applications.

2. Higher cost: Compared to other HPLC columns, the Supelco 59356-U cyano column can be more expensive.

3. Requires careful column maintenance: The cyano stationary phase can be more susceptible to degradation, so proper column maintenance is essential to ensure optimal performance.

The Supelco 59356-U C18 reversed-phasecyano HPLC column (150 mm x 4.6 mm) is an excellent choice for separation of basic and acidic compounds, offering high selectivity, efficiency, and reproducibility. However, its limited sample loading capacity, higher cost, and requiring careful maintenance are potential drawbacks. If you have a need for high-throughput or large-scale separations, you may want to consider a larger diameter column or alternative stationary phases.

For separations of basic and acidic compounds, the Supelco 59356-U C18 reversed-phasecyano HPLC column (150 mm x 4.6 mm) is a reliable and effective choice, especially when high selectivity and efficiency are critical. If you have a large sample volume or require high-throughput analysis, consider a larger diameter column or alternative stationary phases that offer a higher sample loading capacity.

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